Figure 8. Scanning-laser ophthalmoscopy (SLO) imaging. Eyes from Rosa26 (wt; upper row), Rosa26-floxSTOP/SRF-VP16 (het) (middle row), and Rosa26-floxSTOP/SRF-VP16 (hom; bottom row) animals at six months of age (P180) were investigated. Fundus imaging with RF at 514 nm excitation (left column) and autofluorescence at 488 nm laser wavelengths (middle left column) display dramatic changes in fundus appearance, structure of the nerve fiber layer and accumulation of autofluorescent lipids in genetically modified animals compared to wild-type animals. Retinal degenerative processes can also be inferred from enhanced angiographic deep layer (choroidal) signals using fluorescein (middle right column) and indocyanine green (right column) angiography, as clearly detected in both heterozygous and homozygous animals.