Figure 2. Retinal mRNA levels of SRF-VP16 and endogenous Srf at ages P20 and P180. Real-time PCR quantification of relative SRF-VP16 and endogenous Srf mRNA levels in the retinas of P20 and P180 mice. SRF-VP16 RNA levels were only observed in retinas of Rosa26-floxSTOP/SRF-VP16 transgenic mice, both heterozygous (het) and homozygous (hom) for the SRF-VP16 transgene (upper panels). Expression of endogenous Srf was always elevated compared to wild-type mice in the presence of the transgene; this was especially pronounced in the transgenic P180 (hom) mice (lower panels). Error bars indicate SEM, *p<0.05, **p<0.01, and ***p<0.001, denoting significance as compared to wild-type mice. All transcriptional levels were normalized to Gapdh.