Figure 7. Retina-specific promoters are capable of transducing both human retinal pigment epithelial cell line (ARPE19) and 661W cells. Representative images of green fluorescent protein (GFP) expression in ARPE19 cells at 3 days post infection with AAV2 (A) sc-mCARpro-GFP, (B) sc-MOPS500-eGFP, (C) sc-VMD2-GFP, and (D) sc-smCBA-GFP. Representative images of GFP expression in 661W cells at 3 days post infection with AAV2 (E) sc-mCARpro-GFP, (F) sc-MOPS500-eGFP, (G) sc-VMD2-GFP, and (H) sc-smCBA-GFP. All infections were done at a multiplicity of infection (MOI) of 10,000. 10× images A-C and E-G were taken at an ET of 800 ms, whereas images D and H were taken at an ET of 300 ms. The scale bar in A=200 µm.