Figure 5. Transduction efficiency of unmodified scAAV1, scAAV8, and their respective single Y-F capsid mutant vectors. Transduction efficiency of scAAV1 and scAAV1 Y731F (A), scAAV8, scAAV8 Y733F, and scAAV8 Y447F (B) in ARPE19 and 661W cells measured by mCherry-mediated and fluorescence-activated cell sorting (FACS) analysis. Data represents infections at a multiplicity of infection (MOI) of 10,000. mCherry expression is shown in arbitrary units calculated by multiplying the percentage of positive cells by the mean fluorescence intensity in each sample. Each value represents the average of three samples (eight pooled wells of a 24-well plate/sample), based on 10,000 counted cells.