Figure 4. scAAV-mediated mCherry expression in human retinal pigment epithelial (ARPE19) and 661W cone photoreceptor cells, at three days post infection. The top row consists of representative images of mCherry expression in ARPE19 cells infected with (A) scAAV1, (B) scAAV2, and (C) scAAV2 sextuple mutants at a multiplicity of infection (MOI) of 10,000. The bottom row consists of representative images of mCherry expression in 661W cells infected with (D) scAAV8, (E) scAAV2, and (F) scAAV2 sextuple mutants at an MOI of 10,000. All 10× images were taken with identical exposure times (800 ms). The scale bar in A=200 µm.