Figure 3. In vivo transduction pattern of
AAV2 triple and sextuple Y-F capsid mutants. Green fluorescent protein
(GFP) expression in retinal sections of adult mice at one month
following subretinal delivery of (A) AAV2 triple mutant
(Y444F+Y730F+Y500F) or (B) sextuple mutant
(Y444F+Y730F+Y500F+Y272F+Y704F+Y252F) and (C) a comparison of
the relative GFP fluorescence intensity in the retinal pigment
epithelium (RPE) layer of retinas treated with the triple and sextuple
AAV2 vectors, respectively. Values indicate percent GFP intensity
relative to treatment with AAV2 triple mutant vector. All pictures were
evaluated using Image J software. The data are shown as the mean±SEM;
n=3 for each group (p<0.0001). PR represents photoreceptor layer.