Figure 3 of Ryals, Mol Vis 2011; 17:1090-1102.


Figure 3. In vivo transduction pattern of AAV2 triple and sextuple Y-F capsid mutants. Green fluorescent protein (GFP) expression in retinal sections of adult mice at one month following subretinal delivery of (A) AAV2 triple mutant (Y444F+Y730F+Y500F) or (B) sextuple mutant (Y444F+Y730F+Y500F+Y272F+Y704F+Y252F) and (C) a comparison of the relative GFP fluorescence intensity in the retinal pigment epithelium (RPE) layer of retinas treated with the triple and sextuple AAV2 vectors, respectively. Values indicate percent GFP intensity relative to treatment with AAV2 triple mutant vector. All pictures were evaluated using Image J software. The data are shown as the mean±SEM; n=3 for each group (p<0.0001). PR represents photoreceptor layer.