Figure 1. Transduction efficiency of unmodified scAAV vectors in ARPE19 and 661W cells. Cells were infected with scAAV1, 2, 5, 8, and 9 at a multiplicity of infection (MOI) of 10,000. mCherry expression is shown in arbitrary units calculated by multiplying the percentage of positive cells by the mean fluorescence intensity in each sample. Each value represents the average of three samples (eight pooled wells of a 24-well plate/sample), based on 10,000 counted cells.