Molecular Vision: Yang, Mol Vis 2011; 17:1085-1089. Table 1

Table 1. Primers used for candidate gene amplification related with congenital cataract.

Exon	primer (5′-3′)	Product length (bp)	Annealing temperature (°C)
CRYGD-1–2F	AGAACACGAAAATGCCCTTG
CRYGD-1–2R	TGCTTGAAACCATCCAGTGA	579	60
CRYGD-3F	GCTGGACTGCCTAACAATGC
CRYGD-3R	CACATCTTGGTTGCCATTTG	498	58
CRYGS-1F	TTGACTGAAACCAGCCCATA
CRYGS-1R	TTAGGTGAAAAGCGGGTAGG	166	58
CRYGS-2F	AATTAAGCCACCCAGCTCCT
CRYGS-2R	AAGCAAGAGAAAGCGGACAG	356	58
CRYGS-3F	CCTGCTGGTGATTTCCATAA
CRYGS-3R	GATGATGCCTATTTGGACCAC	399	58
GJA8–1F	CGGGGCCTTCTTTGTTCTCTAGTCC
GJA8–1R	AGGCCCAGGTGGCCCAACTCC	750	67
GJA8–2F	CAGCCGGTGGCCCTGCC
GJA8–2R	GTTGCCTGGAGTGCACTGCCC	770	66
GJA3–1F	CGGTGTTCATGAGCATTTTC
GJA3–1R	GACGTAGGTCCGCAGCAG	496	58
GJA3–2F	GCAGGACAATCCCTCGTC
GJA3–2R	GGTCAGGGCTAGCAGTTTGA	532	58
GJA3–3F	TCGGGTTCCCACCCTACTAT
GJA3–3R	TGCACTTTGGTTTTGGTTTC	579	58
CRYAA-1 F	CACGCCTTTCCAGAGAAATC
CRYAA-1 R	CTCTGCAAGGGGATGAAGTG	466	58
CRYAA-2 F	CTTGGTGTGTGGGAGAAGAGG
CRYAA-2R	TCCCTCTCCCAGGGTTGAAG	377	58
CRYAA-3 F	CCCCCTTCTGCAGTCAGT
CRYAA-3R	GCTTGAGCTCAGGAGAAGGA	989	58
CRYGC-1–2F	GCAGTATGTACAGGACAGCGTTA
CRYGC-1–2R	CCTCCCTGTAACCCACATTG	644	58
CRYGC-3F	ATTCCATGCCACAACCTACC
CRYGC-3R	CCCACCCCATTCACTTCTTA	527	58