Figure 1. Upregulation of KLF6 protein by pKLF6 cDNA in rLEC. KLF6 expression can be detected in rLEC’s compared to the vehicle control group and blank control group (lane 1 and 2) in vitro. However, there was increased expression of KLF6 in the experimental group, which indicated that transfection with pKLF6 cDNA can increase expression of KLF6 in rLEC (lane 3) in vitro. glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a loading control.