**Figure 2. **Effect of different inhibitory
conditions on long-term survival of late outgrowth endothelial
progenitor cells and human umbilical vein endothelial cells (HUVECs).
Effect of SU5416 on proliferation of OECs (**A**) and HUVEC (**B**)
in
complete angiogenic medium (EGM-2MV). Cells were grown in EGM-2MV
control conditions (10 μl dimethyl sulfoxide [DMSO]/ml EGM-2MV), or in
the presence of 3, 6, and 10 μM SU5416 added every 48 h. Long-term
survival of OECs (**C** and **E**) and HUVEC (**D** and **F**)
was
similarly assessed in the presence of other VEGFR-2 Tyrosine kinase
inihbitors (TKIs; 10 μM ZM323881, 3 μM KRN951, 4 μM KRN633) and in the
presence of inhibitors of the downstream mediators Akt (100 nM
Wortmannin), PI 3-kinase (5 μM Ly 294002), and PKC (1 μM
bisindolylmaleimide I). All cell counts were performed using the trypan
blue exclusion method and a Neubauer counting chamber. The graphs
represent the mean±SEM cell number for three independent experiments
(OECs derived from three independent (OECs were derived from three
different patients, for HUVEC analysis was repeated at least twice)
experiments. Student’s *t*-test for paired data was used for
statistical comparison between control and inhibitory conditions at
each time point. P values <0.05 were considered significant and are
denoted by an asterisk in the figure).