Figure 7. Immunochemistry of apelin in a retina. Sections of retina were examined by immunohistochemistry with anti-apelin antibody. Positive staining (brown) of apelin was detected in the retinal vessel walls, inner nuclear layer, and outer nuclear layer in the central retinal vein occlusion groups (A: group “1 w,” B: group “2 w,” C: group “24 w”). Among the intravitreal bevacizumab (IVB) groups (D: group “1 w+IVB,” E: group “2 w+IVB,” F: group “24 w+IVB”), only “24 w+IVB” group had positive staining of apelin in nuclear layers.