Figure 1 of Mavlyutov, Mol Vis 2011; 17:1034-1043.


Figure 1. Photoaffinity labeling of the sigma-1 receptor in the retina. Shown are autoradiograms of the photolabeled retinal homogenates that were resolved on sodium dodecyl sulfate gels. Photoaffinity labeling of σRs in the mouse retinal homogenates was performed with [125I]-IAF (lanes 1 and 2). In bovine retinal homogenates, [125I]-IACoc (lanes 3 and 4) and [125I]-IAF (lanes 7 and 8) were used, and labeling was compared to that in rat liver membranes (lanes 5 and 6, 9 and 10, respectively). The specificity of the σR1 photolabeling was demonstrated by the reduced intensity in the presence of the σR1 ligand (+)-pentazocine (the even-numbered lanes). In contrast, the nonspecifically labeled upper bands were not affected by (+)-pentazocine. The typical labeling pattern of the σR1/σR2 bands (seen in lane 9) have often been observed previously in mouse and rat liver membranes [23,30]. In each lane of the gel, 200 μg of total protein was loaded.