Table 1 of Wussuki-Lior, Mol Vis 2011; 17:1011-1015.


Table 1. Primers for PCR amplification of GCNT2, and the Iron Responsive Element (IRE) of the FTL gene.

Gene Annealing temperature Fw primer Rv primer Product size (bp)
GCNT2 exon1A 60 ºC 5’-TGTAGACACAGGTTGCAGGTTAGCA-3’ 5’- GCAGGTAGCTTCATCAAGGGTA -3’ 222 bp
55 ºC 5’-TAGCAGAAGCCTGTCATCAG-3’ 5’- CCTTCAGATACTGAACTATTTC -3’ 491 bp
55 ºC 5’-AACACCTGCGGGCAAGACTT-3’ 5’-CTTTTGTCCTGTGAACAGAGCGGTT-3’ 570 bp
GCNT2 exon1B 55 ºC 5’-AGACTTACAGATTTTGACGGT-3’ 5’-TAGATATTTTGGGGCATGTA-3’ 414 bp
57 ºC 5’-CCATCATCACTTTGACACCT-3’ 5’-CTTATCACATAGGAAAGCTCT-3’ 429 bp
55 ºC 5’-CTCATGCAATTGGACGGACT-3’ 5’-GGGTGAGAACTATATATGTTCCAGTT-3’ 380 bp
GCNT2 exon1C 55 ºC 5’-GCAAATTCAACCTCTCACACCGATC-3’ 5’-GGGGCATATAGATAGCCCTAA-3’ 437 bp
55 ºC 5’-TGTCATGGTCATCCATAAGG-3’ 5’-CTTGGTGGACATATTTAGTT-3’ 407 bp
55 ºC 5’-AGGATTTAAAGGGAAAAATATC-3’ 5’-TGAGTCAGTTCTCTAGGCGAGCAG-3’ 374 bp
GCNT2 exon2 57 ºC 5’-CTGAAGTGGAGAAACCCTGGCTTA-3’ 5’-AACCCTGGATTCCACAGCTACCTT-3’ 514 bp
GCNT2 exon3 55 ºC 5’-AGTTGTAGTTAGTCGGAGAGTACCT-3’ 5’-TATAATTACGTAGCCAGGTCCTGAA-3’ 430 bp
IRE of FTL 58 °C 5’-GGCTGTTAGTGCTCCCATAA-3’ 5’-GATCTGTTCCGTCCAAACAC-3’ 383 bp