Table 1 of Wussuki-Lior, Mol Vis 2011; 17:1011-1015. Table 1 of Wussuki-Lior, Mol Vis 2011; 17:1011-1015.
Table 1. Primers for PCR amplification of GCNT2, and the Iron Responsive Element (IRE) of the FTL gene.
| Gene | Annealing temperature | Fw primer | Rv primer | Product size (bp) |
|---|---|---|---|---|
| GCNT2 exon1A | 60 ºC | 5’-TGTAGACACAGGTTGCAGGTTAGCA-3’ | 5’- GCAGGTAGCTTCATCAAGGGTA -3’ | 222 bp |
| 55 ºC | 5’-TAGCAGAAGCCTGTCATCAG-3’ | 5’- CCTTCAGATACTGAACTATTTC -3’ | 491 bp | |
| 55 ºC | 5’-AACACCTGCGGGCAAGACTT-3’ | 5’-CTTTTGTCCTGTGAACAGAGCGGTT-3’ | 570 bp | |
| GCNT2 exon1B | 55 ºC | 5’-AGACTTACAGATTTTGACGGT-3’ | 5’-TAGATATTTTGGGGCATGTA-3’ | 414 bp |
| 57 ºC | 5’-CCATCATCACTTTGACACCT-3’ | 5’-CTTATCACATAGGAAAGCTCT-3’ | 429 bp | |
| 55 ºC | 5’-CTCATGCAATTGGACGGACT-3’ | 5’-GGGTGAGAACTATATATGTTCCAGTT-3’ | 380 bp | |
| GCNT2 exon1C | 55 ºC | 5’-GCAAATTCAACCTCTCACACCGATC-3’ | 5’-GGGGCATATAGATAGCCCTAA-3’ | 437 bp |
| 55 ºC | 5’-TGTCATGGTCATCCATAAGG-3’ | 5’-CTTGGTGGACATATTTAGTT-3’ | 407 bp | |
| 55 ºC | 5’-AGGATTTAAAGGGAAAAATATC-3’ | 5’-TGAGTCAGTTCTCTAGGCGAGCAG-3’ | 374 bp | |
| GCNT2 exon2 | 57 ºC | 5’-CTGAAGTGGAGAAACCCTGGCTTA-3’ | 5’-AACCCTGGATTCCACAGCTACCTT-3’ | 514 bp |
| GCNT2 exon3 | 55 ºC | 5’-AGTTGTAGTTAGTCGGAGAGTACCT-3’ | 5’-TATAATTACGTAGCCAGGTCCTGAA-3’ | 430 bp |
| IRE of FTL | 58 °C | 5’-GGCTGTTAGTGCTCCCATAA-3’ | 5’-GATCTGTTCCGTCCAAACAC-3’ | 383 bp |