Figure 3. Effects of C5aR antagonist on cell migration, proliferation and EMT in lens capsular bag cultures. A-C: Cell migration of lens epithelial cells treated with control peptide viewed at day 0, 1, 2, and 3, respectively. E: a-SMA staining in control peptide-treated capsular bags 3 days in culture. F-J: Similar series with C5aR antagonist-treated capsular bags. Note that in capsular bags cultured with C5aR antagonist, migration of cells was slower than control. Also note that EMT as was evidenced by a-SMA staining was significantly reduced in the treated cultures (compare E to J). K: BrdU positive cells in both control experimental cultures (statistically significant by t-test; p<0.05).