Figure 1. Effects of C5aR antagonist on EMT of lens epithelial cells cataract surgery in vivo. EMT was assessed by staining of sections with alpha-SMA 1, 2, and 3 weeks after removal of lens fibers. Sections were counter-stained with Hoechst. All sections are compared from the same region (posterior part of the eye as shown by the green rectangle in the illustration). Note the decrease in alpha-SMA signal one week after the operation in the C5aR antagonist-treated animals when compared with the control (A, D; also depicted in the adjacent illustration). Alpha-SMA positive area was gradually decreased even in control animals 2 and 3weeks post-operation (B, C, E, and F) showing similar levels in both groups. 20×.