Table 1 of Preising, Mol Vis 2011; 17:939-948.

Table 1. Sequences and amplification condition for primers designed by the authors.

Name Product Sequence (5′-3′) Annealing temperature Product
size
TYR Primers
Tyr-11f Exon 1, 5′ part CCAATTAGCCAGTTCCTGCAGA 60°, 4% DMSO 345 bp
Tyr-11r   CACAGTTGAATCCCATGAAGTTGC    
Tyr-12f Exon 1, center TATAATAGGACCTGCCAGTGCTCTG 61°, 4% DMSO 342 bp
Tyr-12r   AATGTCTCTCCAGATTTCAGATCCC    
Tyr-13f Exon 1, 3′ part TGTGTCAATGGATGCACTGCTT 60°, 4% DMSO 331 bp
Tyr-13r   AGAAGTGATTGTTAAGGTTCCTCCC    
Tyr-2f Exon 2 TTGTTTAACATGAGGGTGTTTTGTACAG 60°, 4% DMSO 313 bp
Tyr-2r   GGACTTTGGATAAGAGACTGTAAATATG    
Tyr-3f Exon 3 ATAATTATAAATCAATCACATAGGTTTTCA 55° 263 bp
Tyr-3r   CCAATGAGCACGTTATTTATAAAGA    
Tyr-4f Exon 4 AAAATTTTCAAATGTTTCTTTTATACACA 56°, 4% DMSO 280 bp
Tyr-4r   CAGCAATTCCTCTGAAAGAAAGTAA    
Tyr-5fa Exon 5 TGAAAGGATGAAGATGATGGTGATC 61°, 4% DMSO 350 bp
Tyr-5ra   TTGAGTTAGAGTGAGGTCAGGCTTTT    
OCA2 Primers
PG2f Exon 2 AGTGGTTTCTTTCTGGCTGCCC 60° 313 bp
PG2r   TGAAGTCCACATTTACAAGATGGCA    
PG251f Exon 25 TCTCATGAGCTTATCCAGATTTCAGA 60° 222 bp
PG251r   GTGGGGTCAGGGTAGTTTTATGACTA    
MC1R Primers
MC1Rf 5′ sense ACTTAAAGCCGCGTGCACCG 65°, 4% DMSO 1016 bp
MC1Rr 3′ antisense AGGCCTCCAACGACTCCTTCCT    
Sequencing primers
MC1Ria internal sense GGTGCTGCAGCAGCTGGACAAT    
MC1Rib internal antisense AGAAGACCACGAGGCACAGCAGGAC    

All amplifications were run in a program of 94 °C denaturation for 5 min, 35 cycles of annealing (Tann see in table), elongation (72 °C), and denaturation (94 °C) for 1 min and completed for a final annealing at 1 min and elongation for 10 min closing with 10 min at 10 °C. aOligonucleotides used to amplify exon 5 of TYR also amplify exon 5 of the TYR-pseudogene.

Preising, Mol Vis 2011; 17:939-948. http://www.molvis.org/molvis/v17/a104

©2011 Molecular Vision http://www.molvis.org/molvis/

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