Molecular Vision: Gao, Mol Vis 2011; 17:903-919. Table 1

Table 1. Primers used to examine gene expression using qPCR.

	Primer sequence
Gene	Forward	Reverse	Size	Spans intron	Efficiency
Signaling pathways
TGFB1	ACCAGAAATACAGCAACAATTCC	AACCCGTTGATGTCCACTTG	205	Yes	98
TGFB2	GCAGAGTTTAGGGTCTTTCGTTTG	CTCGTGAACAGCATCAGTTACATC	189	Yes	104
TGFB3	ATCACCATAACCCGCATCTAATCC	CGCACACAGCAGTTCTCCTC	139	Yes	91
TGFBR1	GACCTCCCAACTACTGTAAAGC	ATCCTCTTCATTTGGCACTCG	162	Yes	89
TGFBR2	GCTGCCTGTGTGACTTTGG	TCCTGGATTCTAGCACTTCTGG	123	Yes	94
TGFBR3	CCCTGGTCTGGCGTCTGAAG	GTAACTGCTCCATACTCGTTTCGG	190	Yes	87
FGF2	GGGTCGTGTCTATCAAAGGAG	ACATTTAGAAGCCAGCAGTCG	80	Yes	90
FGFR1	CCTGGAGGTCATCATCTACTGC	AGAGTTCATGGAAGCACTGGAG	196	Yes	96
APOE	GGTGCAGACGCTGTCTGACCA	CCTCCAACTCCGCCTTGTAGG	122	Yes	89
APOA1	GCTGTGGTATTGACCTTGGCTGT	TTGGCTAAATCCCGCACTCG	110	Yes	100
Matricellular proteins
THBS1	CTGTCAGAACTCAGTCACCATC	CCACGGAGACCAGCCATC	136	Yes	81
THBS2	GAGACCGACTTCAGGAACTTC	CGAAACCCACTGCGATGC	142	Yes	88
TNC	AGACGCCAAGACTCGCTACAG	CAGGTTGACACGGTGACAGTTC	184	Yes	88
SPARC	GCGAGTTTGAGAAGGTGTGC	GCCCGATGTAGTCCAGGTG	126	Yes	78
SPP1	CCGACGACACCGACCATCC	GGCTTTGACCTCACTCTGTAAACC	190	No	83
MP/TIMP
MMP3	GCCATCCGAGGAAATGAGG	TGTCTCTTCTCGTCAAATCTCC	164	Yes	95
MMP14	CCCTGGAACCTGGCTACCC	ATAGGTCTTTCCATTGGGCATCC	104	No	104
ADAMTS1	TGGCAAAAGCAGCACAACCC	CACAGGTCTGAGCCCCACAC	100	Yes	92
ADAMTS5	TCTTCCATCCTAACCAGCATTG	GGTGGCATCATAAGTCTGTCC	165	Yes	105
TIMP3	CCGTGTCTATGATGGCAAGATG	ACAAAGCAAGGCAGGTAATAGC	153	Yes	82
Cell adhesion/other
SDC2	TGATGACGACTACGCTTCTGC	CAGGCATCTTGTTCTGTGTCTTC	155	Yes	90
SDC4	CAAGGAACTGGAGGAGAATG	GGAAAGTGGCAAAGAGGAG	182	Yes	80
VIM	GCTCACCAACGACAAGGC	CAGAGTGCTTTCGGCTTCC	121	Yes	94
COL6A1	CGACATCCTGTTCGTGCTGG	ATCTGGTTGTGGCTGTACTGC	168	Yes	78
FN1	GCAACTCATCAGCATCCAGCAATATG	GGAAACCCAGGAGACCACAAAGC	190	Yes	84
PEDF	CCTGAAAGCAACCCAGAACTTGA	GACTTGGTAACTTCGCCTTCGTAAC	147	Yes	90
TGFBI	CCTCGGCACTCATCTCTCC	GCAAATTCTTCATCTTGGCATCG	107	Yes	96
Reference
POLR2A	CTACCAGCCCCAAGTATTC	GGTGAGTAAGTAGGAGACG	106	No	101