Figure 5. Expression of LRP5.WT and
LRP5.Q89R was not different in transiently transfected GTM3 cells. GTM3
cells were transiently transfected with empty, LRP5.WT-MycHis or
LRP5.Q89R-MycHis expression vector. Whole cell lysate was analyzed by
western immunoblotting (A). LRP5.WT and LRP5.Q89R fusion
proteins were detected with an anti-cMyc antibody. GADPH served as a
loading control. B: The expression levels of LRP5.WT and
LRP5.Q89R were not statistically different. The optic density of LRP5
protein bands was first normalized to GAPDH and then normalized to
LRP5.WT. Each column represents the mean±SD of normalized LRP5
expression of eight samples (LRP5.WT 100%±0 versus LRP5.Q89R 109%±18%;
n=8). NS: not significant, p>0.05.