Figure 5 of Mao, Mol Vis 2011; 17:894-902.


Figure 5. Expression of LRP5.WT and LRP5.Q89R was not different in transiently transfected GTM3 cells. GTM3 cells were transiently transfected with empty, LRP5.WT-MycHis or LRP5.Q89R-MycHis expression vector. Whole cell lysate was analyzed by western immunoblotting (A). LRP5.WT and LRP5.Q89R fusion proteins were detected with an anti-cMyc antibody. GADPH served as a loading control. B: The expression levels of LRP5.WT and LRP5.Q89R were not statistically different. The optic density of LRP5 protein bands was first normalized to GAPDH and then normalized to LRP5.WT. Each column represents the mean±SD of normalized LRP5 expression of eight samples (LRP5.WT 100%±0 versus LRP5.Q89R 109%±18%; n=8). NS: not significant, p>0.05.