Figure 4. The canonical Wnt signaling activity in HEK293T cells after LRP5 overexpression. HEK293T cells were transiently transfected with TCF luciferase plasmids and empty, LRP5.WT, or LRP5.Q89R expression vectors. A: Wnt signaling activity after treatment with or without 50 ng/ml recombinant Wnt3a. B: DKK1 inhibition of Wnt signaling. HEK293T cells were also co-transfected with or without 10 ng DKK1 expression vector. Cells were treated with or without 100 ng/ml recombinant Wnt3a. Each column represents the mean±SD of relative luminescence unit (RLU; ×1000) of three samples (n=3). *: p<0.05 **: p<0.01. NS: not significant, p>0.05.