Figure 2. Expression of LRP5.WT and
LRP5.Q89R was not different in transiently transfected HEK293T cells.
HEK293T cells were transiently transfected with empty, LRP5.WT-MycHis
or LRP5.Q89R-MycHis expression vector. Whole cell lysate was analyzed
by western immunoblotting (A). LRP5.WT and LRP5.Q89R fusion
proteins were detected with an anti-cMyc antibody. GADPH served as a
loading control. (B) The expression levels of LRP5.WT and
LRP5.Q89R were not statistically different. The optic density of LRP5
protein bands was first normalized to GAPDH and then normalized to
LRP5.WT. Each column represents the mean±SD of normalized LRP5
expression of five samples (LRP5.WT 100%±0 versus LRP5.Q89R 111%±15%;
n=5). NS: not significant, p>0.05.