Table 1 of Wang, Mol Vis 2011; 17:70-77. Table 1 of Wang, Mol Vis 2011; 17:70-77.
Table 1. Primer sequences for PCR amplification.
Gene |
Forward primers (5′→3′) |
Reverse primers (5′→3′) |
Annealing temperature (°C) |
Product size (bp) |
|---|---|---|---|---|
| CRYAA-1 | AGCAGCCTTCTTCATGAGC | CAAGACCAGAGTCCATCG | 62 | 584 |
| CRYAA-2 | GGCAGGTGACCGAAGCATC | GAAGGCATGGTGCAGGTG | 62 | 550 |
| CRYAA-3 | GCAGCTTCTCTGGCATGG | GGGAAGCAAAGGAAGACAGA | 62 | 511 |
| CRYBA1-1 | GGCAGAGGGAGAGCAGAGTG | CACTAGGCAGGAGAACTGGG | 60 | 550 |
| CRYBA1-2 | AGTGAGCAGCAGAGCCAGAA | GGTCAGTCACTGCCTTATGG | 60 | 508 |
| CRYBA1-3 | AAGCACAGAGTCAGACTGAAGT | CCCCTGTCTGAAGGGACCTG | 60 | 463 |
| CRYBA1-4 | GTACAGCTCTACTGGGATTG | ACTGATGATAAATAGCATGAACG | 60 | 355 |
| CRYBA1-5 | GAATGATAGCCATAGCACTAG | TACCGATACGTATGAAATCTGA | 60 | 597 |
| CRYBA1-6 | CATCTCATACCATTGTGTTGAG | CATCTCATACCATTGTGTTGAG | 62 | 528 |
| CRYBB1-1 | CCCTGGCTGGGGTTGTTGA | TGCCTATCTGCCTGTCTGTTTCTC | 58 | 620 |
| CRYBB1-2 | TAGCGGGGTAATGGAGGGTG | AGGATAAGAGTCTGGGGAGGTGG | 58 | 664 |
| CRYBB1-3 | CCTGCACTGCTGGCTTTTATTTA | TCTCCAGAGCCCAGAACCATG | 60 | 475 |
| CRYBB1-4 | CCAACTCCAAGGAAACAGGCATA | CCTCCCTACCCACCATCATCTC | 60 | 491 |
| CRYBB1-5 | TAGACAGCAGTGGTCCCTGGAGA | AGCACTGGGAGACTGTGGAAGG | 60 | 416 |
| CRYBB1-6 | CCTAGAAAAGGAAACCGAGGCC | AGCGAGGAAGTCACATCCCAGTA | 60 | 551 |
| CRYBB2-1 | GTTTGGGGCCAGAGGGGAGTGGT | TGGGCTGGGGAGGGACTTTCAGT | 62 | 349 |
| CRYBB2-2 | CCTTCAGCATCCTTTGGGTTCTCT | GCAGTTCTAAAAGCTTCATCAGTC | 62 | 330 |
| CRYBB2-3 | GTAGCCAGGATTCTGCCATAGGAA | GTGCCCTCTGGAGCATTTCATAGT | 62 | 360 |
| CRYBB2-4 | GGCCCCCTCACCCATACTCA | CTTCCCTCCTGCCTCAACCTAATC | 62 | 230 |
| CRYBB2-5 | CTTACCCTTGGGAAGTGGCAATGG | TCAAAGACCCACAGCAGACAAGTT | 62 | 600 |
| CRYGC-1 | TGCATAAAATCCCCTTACCG | CCTCCCTGTAACCCACATTG | 62 | 514 |
| CRYGC-2 | TGGTTGGACAAATTCTGGAAG | CCCACCCCATTCACTTCTTA | 60 | 430 |
| CRYGD-1 | CAGCAGCCCTCCTGCTAT | GGGTCCTGACTTGAGGATGT | 60 | 550 |
| CRYGD-2 | GCTTTTCTTCTCTTTTTATTTCTGG | AAGAAAGACACAAGCAAATCAGT | 62 | 308 |
| CX46-1 | CGGTGTTCATGAGCATTTTC | CTCTTCAGCTGCTCCTCCTC | 60 | 450 |
| CX46-2 | GAGGAGGAGCAGCTGAAGAG | AGCGGTGTGCGCATAGTAG | 60 | 450 |
| CX46-3 | TCGGGTTCCCACCCTACTAT | TATCTGCTGGTGGGAAGTGC | 62 | 300 |
| CX50-1 | CCGCGTTAGCAAAAACAGAT | CCTCCATGCGGACGTAGT | 62 | 420 |
| CX50-2 | GCAGATCATCTTCGTCTCCA | GGCCACAGACAACATGAACA | 62 | 330 |
| CX50-3 | CCACGGAGAAAACCATCTTC | GAGCGTAGGAAGGCAGTGTC | 62 | 350 |
| CX50-4 | TCGAGGAGAAGATCAGCACA | GGCTGCTGGCTTTGCTTAG | 62 | 500 |
| MIP-1 | GTGAAGGGGTTAAGAGGC | GGAGTCAGGGCAATAGAG | 62 | 561 |
| MIP-2 | CGGGGAAGTCTTGAGGAG | CACGCAGAAGGAAAGCAG | 58 | 847 |
| MIP-3 | CCACTAAGGTGGCTGGAA | CTCATGCCCCAAAACTCA | 60 | 561 |