Table 1 of Roshan, Mol Vis 2010; 16:887-896. Table 1 of Roshan, Mol Vis 2010; 16:887-896.
Table 1. List of primers used for PCR amplification of lens specific genes.
| Primer | Exon | Primer sequence 5’->3’ | Product size (bp) |
|---|---|---|---|
| CRYAA-1F | 1 | CTCCAGGTCCCCGTGGTACCA | 254 |
| CRYAA-1R | GCGAGGAGAGGCCAGCACCAC | ||
| CRYAA-2F | 2 | CTGTCTCTGCCAACCCCAGCAG | 223 |
| CRYAA-2R | CCCCTGTCCCACCTCTCAGTGCC | ||
| CRYAA-3F | 3 | GGCAGCTTCTCTGGCATGGGG | 312 |
| CRYAA-3R | GGGGAGCCAGCCGAGGCAATG | ||
| CRYBB2-1F | 1 | TCTGTGGGCATTTGCTGACCC | 292 |
| CRYBB2-1R | GCTAACAGCATTGAAGTCTCTGCCC | ||
| CRYBB2-2F | 2 | GACCCCACAGCTCTGGGACAGTC | 393 |
| CRYBB2-2R | GGAGGGACTTTCAGTATCAGCTCCAAC | ||
| CRYBB2-3F | 3 | CACGGCTGCTTATAGCCAGAGCC | 449 |
| CRYBB2-3R | TCTATCTGACTGCAAAGCATGAATTATCTCC | ||
| CRYBB2-4F | 4 | GCTTTGGGCACAGCGATGTTCTG | 744 |
| CRYBB2-4R | GGCCCCTTCCTGGTCCCCA | ||
| CRYBB2-5F | 5 | AGTGGTCATAGACACGTAGTGGGTGCAC | 706 |
| CRYBB2-5R | CTGTTCCCAAACTTAGGGACACACGC | ||
| CRYBB2-6F | 6 | CCCCTCGTTCACCCTCCCATCA | 506 |
| CRYBB2-6R | CACTGTGTCCAAGGTCACACAGCTAAGC | ||
| CRYGA-2F | 1&2 | AGGTCCCTTTTGTGTTGTTTTTGCC | 462 |
| CRYGA-2R | CATGAGGAATTATACGGCAGGATTGG | ||
| CRYGA-3F | 3 | CAGACCAGCTCGCACAAGTTAAGG | 353 |
| CRYGA-3R | AAGAGCCACTTAGTGCAGGGAACACAAC | ||
| CRYGB-2F | 1&2 | TGCAAATCCCCTACTCACCAAAATGG | 518 |
| CRYGB-2R | AAAAAGATGGAAGGCAAAGACAGAGCC | ||
| CRYGB-3F | 3 | TTTGTTTACTCTTGCGTTTTCTGTCTGCC | 410 |
| CRYGB-3R | GAAAGAAAGACAGGGCTCTACTAGTGCC | ||
| CRYGC-2F | 1&2 | TGCATAAAATCCCCTTACCGCTGAG | 522 |
| CRYGC-2R | ACTCTGGCGGCATGATGGAAATC | ||
| CRYGC-3F | 3 | AGACTCATTTGCTTTTTTCCATCCTTCTTTC | 407 |
| CRYGC-3R | GAAAGAATGACAGAAGTCAGCAATTGCC | ||
| CRYGD-2F | 1&2 | GCAGCCCCACCCGCTCA | 599 |
| CRYGD-2R | GGGTAATACTTTGCTTATGTGGGGAG | ||
| CRYGD-3F | 3 | TGCTTTTCTTCTCTTTTTATTTCTGGGTCC | 400 |
| CRYGD-3R | AGTAAAGAAAGACACAAGCAAATCAGTGCC | ||
| GJA8-L1F | 1 | CGGGGCCTTCTTTGTTCTCTAGTCC | 877 |
| GJA8-L1R | AGGCCCAGGTGGCTCAACTCC | ||
| GJA8-L2F | 1 | CAGCCGGTGGCCCTGCC | 907 |
| GJA8-L2R | GTTGCCTGGAGTGCACTGCCC | ||
| GJA3-1aF | 1 | CTGCGATGCCTGTCCTGTGG | 539 |
| GJA3-1aR | TTGTCCTGCGGTGGCTCCTT | ||
| GJA3-1bF | 1 | CGCCCACCCTCATCTACCT | 549 |
| GJA3-1bR | GTGGGAACCCGATGGCAAC | ||
| GJA3-1cF | 1 | AGCTCAAGCAGGGCGTGACC | 542 |
| GJA3-1cR | CAAGGGCGGCTGGTGCATCT | ||
| GJA3-1dF | 1 | CCCCGGCGCTCAAGGCTTAC | 545 |
| GJA3-1dR | AACCCTTGTCCCCGCCACCC | ||
| PAX6-1F | 1 | CTCATTTCCCGCTCTGGTTC | 300 |
| PAX6-1R | AAGAGTGTGGGTGAGGAAGT | ||
| PAX6-2F | 2 | CACACTCTTTATCTCTCACTCTCCAGCC | 300 |
| PAX6-2R | AATAAAGCGAGAAAGAAGCGGAC | ||
| PAX6-3F | 3 | TCAGAGAGCCCATCGACGTAT | 300 |
| PAX6-3R | CTGTTTGTGGGTTTTGAGCC | ||
| PAX6-4F | 4 | TTGGGAGTTCAGGCCTACCT | 153 |
| PAX6-4R | GAAGTCCCAGAAAGACCAGA | ||
| PAX6-5F | 5 | CCTCTTCACTCTGCTCTCTT | 257 |
| PAX6-5R | ATGAAGAGAGGGCGTTGAGA | ||
| PAX6-5aF | 5a | TGAAAGTATCATCATATTTGTAG | 237 |
| PAX6-5aR | GGGAAGTGGACAGAAAACCA | ||
| PAX6-6F | 6 | GTGGTTTTCTGTCCACTTCC | 299 |
| PAX6-6R | AGGAGAGAGCATTGGGCTTA | ||
| PAX6-7F | 7 | CAGGAGACACTACCATTTGG | 252 |
| PAX6-7R | ATGCACATATGGAGAGCTGC | ||
| PAX6-8F | 8 | GGGAATGTTTTGGTGAGGCT | 371 |
| PAX6-8R | CAAAGGGCCCTGGCTAAATT | ||
| PAX6-9F | 9 | GTAGTTCTGGCACAATATGG | 206 |
| PAX6-9R | GTACTCTGTACAAGCACCTC | ||
| PAX6-10F | 10 | GTAGACACAGTGCTAACCTG | 243 |
| PAX6-10R | CCCGGAGCAAACAGGTTTAA | ||
| PAX6-11F | 11 | TTAAACCTGTTTGCTCCGGG | 208 |
| PAX6-11R | TTATGCAGGCCACCACCAGC | ||
| PAX6-12F | 12 | GCTGTGTGATGTGTTCCTCA | 300 |
| PAX6-12R | TGCAGCCTGCAGAAACAGTG | ||
| PAX6-13F | 13 | CATGTCTGTTTCTCAAAGGGA | 957 |
| PAX6-13R | GAACAATTAACTTTTGCTGGCC |