Figure 5. The production of interleukin-17 and interferon-γ by polyclonally stimulated peripheral blood mononuclear cells from patients with Behcet’s disease before and after one- and three- month cyclosporin A treatment. A: Levels of IL -17 in supernatants of peripheral blood mononuclear cells from BD patients (n=6) before and after treatment and normal controls (n=6) were determined by ELISA. IL-17 production by PBMCs was significantly downregulated in BD patients after CsA treatment compared with that before treatment. B: The frequency of IL-17-producing T cells from BD patients before and after treatment and normal controls was determined by FACS. Both IL-17-producing CD4⁺ and CD8⁺ T cells were significantly decreased in BD patients at 1 and 3 months after CsA treatment compared with those before treatment. C: Concentrations of IFN-γ in supernatants of PBMCs from BD patients before and after treatment and normal controls were determined by ELISA. IFN-γ production by polyclonally stimulated PBMCs was significantly lower in BD patients after CsA treatment than before treatment. D: The frequency of IFN-γ-producing T cells from BD patients before and after treatment and normal controls were determined by FACS. Both IFN-γ-producing CD4⁺ and CD8⁺ T cells were significantly decreased in BD patients after CsA treatment compared with those before treatment. Data are shown representative of five independent experiments.