Figure 3. Effect of small interfering RNA on the expression of epithelial cell adhesion molecule in retinoblastoma Y79 cells in vitro.
Immunofluorescence picture shows the negative staining without Epithelial cell adhesion molecule (Ep-CAM) antibody in Y79
cells representing as negative control (A); Immunofluorescence shows strong positivity (3+; white arrows) for Ep-CAM on day 8 post azacytidine treatment (B); After 24 h of short interfering (si)RNA treatment, Y79 cells showed moderate staining (2+; arrows) of Ep-CAM (C). After 48 h of siRNA treatment, Y79 cells showed dull staining (1+; arrows) of Ep-CAM (D). After 72 h of siRNA treatment, Y79 cells gain Ep-CAM expression (low to moderate intensity). E: western blot analysis demonstrates strong expression of Ep-CAM in Y79 cells that are si-RNA untreated (lane 1), strong expression
in Y79 cells treated with scrambled siRNA (lane 2) and markedly reduced Ep-CAM expression in Y79 cells treated with Ep-CAM-siRNA
(F; lane 3).