Figure 1. Apoptosis and mitosis in human lens epithelial cells (HLEC) following incubation with siramesine. Cells were stained with Hoechst and the nuclear morphology was examined by confocal microscopy. The diagram shows the average percentage of cells in three independent wells displaying apoptotic and mitotic nuclei after 1–4 h of incubation with siramesine or solvent (0.1% DMSO; C). A clear proapoptotic effect of siramesine was observed already after 4h. A tendency for a reduced mitotic activity was observed at this time. Results are displayed with mean±SEM, p<0.001 (***). The experiment was repeated three times with similar results.