Figure 4 of Chee, Mol Vis 2010; 16:800-812.


Figure 4. Effect of osmotic stress on the subcellular distribution of NCC and NKCC1 in the cortex of the rat lens. Images from equatorial cryosections obtained from lenses organ cultured in either isotonic, hypotonic or hypertonic AAH, and doubled-labeled with either, NKCC1 or NCC specific antibodies (green), and the membrane marker TRITC-WGA (red). A: Overview image of fiber cell morphology in the lens cortex taken from a lens incubated under isotonic conditions to indicate the relative locations of the efflux and influx zones. B, C: Representative high power images of NCC (B) and NKCC1 (C) labeling taken from the efflux (top panels) and influx (lower panels) zones from lenses incubated for 18 h in isotonic (left column), hypotonic (middle column), and hypertonic (right column) AAH for 18 h.