Figure 2. Sequence analysis and amino acid conservation of the mutated cyclic nucleotide-gated channel alpha-3 (CNGA3) amino acid residue in family RP26. A: Sequence trace of part of exon 8 in a normal control individual (left panel) and an affected individual (V-2; right panel) showing the homozygous mutant sequence c.822G>T (p.R274S). B: Protein sequence alignment of CNGA3 in different species. The mutated arginine residue is highly conserved. The conserved amino acids between different species are shown in black boxes, and the less conserved amino acids are highlighted in light gray or white boxes.