Figure 5. Immunofluorescence analysis of choroidal vascularity in rat eyes after topical thioredoxin-vasostatin 48 application. The
choroidal vascularity in thioredoxin (TRX)-treated or TRX-fused vasostatin 48 (TRX-VS48)-treated eyes was examined by immunofluorescence
analysis using anti-Von Willebrand factor (vWF). A: Representative profiles of vWF-positive vessels in retinochoroid of TRX-treated or TRX-VS48-treated eyes are shown. Arrows
indicate big circles in high power field (larger arrows) and choroidal neovascularizationby little circles in low power field
(arrowheads). B: Quantification pf vWF-positive blood vessels in choroids from TRX-VS48- or TRX-treated eyes. Data are mean±SEM (n=8) *(p<0.01).