Figure 3. Flat-mount analysis of choroidal vascularity detected by fluorescein isothiocyanate-dextran staining in rat eyes after topical thioredoxin-vasostatin 48 application. The choroidal vascularity of laser-induced lesions in rat eyes was examined by injecting fluorescein isothiocyanate (FITC)-dextran. A: FITC-dextran positive blood vessels are presented in choroids of thioredoxin(TRX)-treated or TRX-fused vasostatin (TRX-VS48)-treated rats. Arrows indicate the laser-induced lesions. B: Quantification of FITC-dextran fluorescence in choroids of TRX-treated eyes or TRX-VS48-treated eyes are shown. Data are summarized as mean±SEM (n=8). Double asterisk (**) represents p<0.01.