Figure 1. Effect of thioredoxinand thioredoxin-vasostatin 48 on angiogenic processes in cultured endothelial cells. A: Scheme represented thioredoxin (TRX) and TRX-fused VS48 (TRX-VS48). The purity of TRX and TRX-VS48 was analyzed by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) analysis. Recombinant TRX-VS48 is expressed and purified with a molecular weight of 26 kDa (arrow). B: Effect of TRX-VS48 on endothelial cell migration was assayed by the Boyden's chamber method. After treatment with TRX-VS48, the cell migration of ECV304 cells was significantly decreased. C: Tube formation of ECV304 cells was quantified on matrigel plates. After treatment with TRX-VS48, the tube formation of ECV304 cells was significantly decreased. Data represents the mean±SEM of quadruplicate experiments. *p<0.05.