Figure 2. Representative image showing AT1 and AT2 mRNA expression in rabbit corneal epithelium detected with PCR. Appropriate size amplification products for AT1 (463 bp) and AT2 (551 bp) were detected in two independent rabbit corneal epithelium cDNA samples prepared from different rabbits. No signal
for ACE was detected. -ve con denotes negative controls that contained ACE, AT1, or AT2 primers but water instead of cDNA. + ve con represents the positive control of β-actin (ACTB; 350 bp). L denotes 1 kb plus the DNA ladder.