Figure 1. Representative image showing detection of ACE, AT₁, and AT₂ mRNA expression in rabbit corneal fibroblasts with PCR. Appropriate size amplification products for ACE (469 bp), AT₁ (463 bp), and AT₂ (551 bp) were detected in two independent cDNA samples of corneal fibroblast prepared from different rabbits. -ve con denotes negative controls that contained ACE, AT₁, or AT₂ primers but water instead of cDNA. + ve con represents the positive control of β-actin (ACTB; 350 bp). L denotes 1 kb plus the DNA ladder.