Figure 2. Trabecular meshwork cells express zonula occludens 1 and Cx43. NTM cells have lower zonula occludens 1 (ZO-1) α– isoform levels but higher connexin 43 (Cx43) levels compared to GTM cells, yet NTM and GTM cells have similar ZO-1 α+ levels, as illustrated by RT–PCR. GAPDH was used as the internal loading control. M stands for molecular size ladder. n=3.