Table 1 of Jia, Mol Vis 2010; 16:676-681. Table 1 of Jia, Mol Vis 2010; 16:676-681.
Table 1. Oligonucleotides used for PAX6 amplification.
Exon |
Sequences (5′-3′) |
Annealing temperature (°C) |
|---|---|---|
| 4 | F:TGATGCAGCTGCCCGAGGATTA | 68 |
| R:GGGGCGAGAGGGGGTGTGAGTTA | ||
| 5 | F:TTCCCCTTCCTCCTCTCCTTTCT | 62.4 |
| R:GGGGGTCCATAATTAGCATCGTT | ||
| 6 | F:GCAAGGTCAGCACAAAAATAAAT | 66.5 |
| R:TGCCCTGGGTCTGATGGA | ||
| 7 | F:AAAACGTAAGCTTGTCATTGT | 58 |
| R:GAGAGGGTGGGAGGAGGTA | ||
| 8 | F:TTCCATGCCCAAAGTGATAG | 60 |
| R:TTCCCAGGCCAACAAAAT | ||
| 9 | F:AAAATGGTGGTCAGGTAACTAAC | 62.4 |
| R:TGAAAAGATGCCCAGAGAAATAA | ||
| 10 | F:TTGGTTGGAGGTAATGGGAGTG | 62.4 |
| R:GGAAATCAGGTGGGACAGGTTAG | ||
| 11 | F:CGTGGGGAGGGCAGCAGTG | 68 |
| R:ATGGAGCCAGATGTGAAGGAGGAA | ||
| 12 | F:GAAGGGCCAAATGGAGAAGAGA | 68 |
| R:TGCAGACACAGCCAATGAGGT | ||
| 13 | F:GACTAGCTCGAGGCCCAATCTTA | 64.8 |
| R:TTCCCTTTTCAATCCCCATCC | ||
| 14 | F:TTGCTGGAAATAAAAAGTGA | 58.9 |
| R:CGGCTCTAACAGCCATTTTT | ||
| H-SSCP analysis of exon 5. | F:TTCTCCCTCATCTTCCTCTTCCTT | 64.8 |
| R:GCAGTGGCCGCCCGTTGAC |