Figure 1. Reverse transcriptase PCR analysis of transient receptor potential vanilloid member 5 (TRPV5) and member 6 (TRPV6) expression in cultured human retinal pigment epithelium. Agarose gel electrophoresis of PCR products amplified with TRPV5- (lane 2) and TRPV6- (lane 1) specific primers. CDNA (cDNA) was from primary retinal pigment epithelium cultures established from donor tissue from a 10-year-old Caucasian female. Arrows to the right identify the expected size of the PCR DNA for TRPV5 (312 bp) and for TRPV6 (301 bp). Direct sequencing of the purified PCR DNAs confirmed the identities of the bands as TRPV5 and TRPV6 transcripts.