Table 2. Quantitative RT–PCR analysis of mRNA expression for
the natriuretic peptides and receptors in HLE-B3 cells.
mRNA expression levels were measured by quantitative RT–PCR in an
experiment in which HLE-B3 cells were subjected to serum deprivation.
Independent triplicate populations of cells were collected. Analysis of
quantitative RT–PCR data was based upon calculating ΔCT
values using a β-actin control and a baseline standard of mRNA
expression at 20% FBS MEM. Values represent an n=6 and are expressed
relative to the respective baselines (20% FBS MEM) which are assigned a
value of 1.0. Values represent the mean±standard error.