Table 1 of Cammarata, Mol Vis 2010; 16:630-638. Table 1 of Cammarata, Mol Vis 2010; 16:630-638.
Table 1. Natriuretic peptide and receptor oligonucleotide primer pairs.
Gene (GeneBank accession) |
Primer sequence (Forward/Reverse) |
Location |
PCR product (bp) |
|---|---|---|---|
| Actin, beta (ACTB; NM_001101) | 5′-CATCCTCACCCTGAAGTACC-3′
5′-GTACAGGGATAGCACAGCCT-3′ |
273–513 | 241 |
| Natriuretic peptide precursor A (NPPA; NM_006172) | 5′-GATTTCAAGAATTTGCTGGACCAT-3′ 5′-TTGCTTTTTAGGAGGGCAGATC-3′ |
209–435 | 227 |
| Natriuretic peptide receptor A (NPR-A; NM_000906.3) | 5′-GCAAAGGCCGAGTTATCTACATC-3′ 5′-AACGTAGTCCTCCCCACACAA-3′ |
1131–1228 | 98 |
| Natriuretic peptide precursor B (NPPB; NM_002521) | 5′-CGGGTTACAGGAGCAGCG-3′ 5′-CTCCAGGGATGTCTGCTCCA-3′ |
225–297 | 73 |
| Natriuretic peptide receptor B (NPR-B; NM_003995) | 5′-CGGGAGGATGGACTTCGA-3′
5′-CATGACAACCAGCCCAGTTACA-3′ |
1072–1146 | 75 |
| Natriuretic peptide precursor C (NPPC; NM_024409) | 5′-AGCGTGGGCTCGCCTT-3′ 5′-CTTGTTGGCTCCTTTGTATTTGC-3′ |
249–309 | 61 |
| Natriuretic peptide receptor C (NPR-C; NM_000908) | 5′-GGAAGACATCGTGCGCAATA-3′ 5′-GATGCTCCGGATGGTGTCA-3′ |
950–1028 | 79 |