Figure 5. eNOS production in human lens epithelial cell lines HLE-B3 and SRA 01/04. Protein expression for eNOS was measured by western blot analysis with HLE-B3 and SRA 01/04 cells subjected to hypoxia followed by reintroduction of oxygen. Western blots were run with an actin control and the lanes were equally loaded at a concentration of 10 µg protein per lane. Note that authentic eNOS standard was detected with the commercial antibody.