Figure 2. Immunoflourescent analysis of natriuretic peptide receptor expression in HLE-B3 cells. Immunoflourescence and confocal microscopy was used to determine expression of natriuretic peptide receptors on HLE-B3 cells. Control images included HLE-B3 cells incubated with no primary antibody but with a goat-anti-rabbit secondary and cells incubated with rabbit IgG as the primary antibody. The data are typical of images representative of eight random fields of view per receptor taken from two independent populations of cells. The scale bar equals 50 microns.