Figure 1. Reverse Transcriptase-PCR analysis of the natriuretic peptides (ANP, BNP, and CNP) and receptors (NPR-A, NPR-B, and NPR-C) in HLE-B3 cells. Total RNA was isolated from HLE-B3 cells and subjected to RT–PCR for cDNA synthesis. Polymerase chain reactions
were run with the synthesized cDNA with the primer pairs for each of the three natriuretic peptides and natriuretic peptide
receptors. PCR products were run on 2.5% agarose gel alongside a 100 bp step ladder (Promega Corporation, Madison, WI). All
products resolved in the expected location.