Table 2 of Lu, Mol Vis 2010; 16:611-622. Table 2 of Lu, Mol Vis 2010; 16:611-622.
Table 2. Primer pairs used in reverse transcription-polymerase chain reaction detection. Keratin 3 (K3), neuron-specific enolase (NSE), VDAC3, CLCN3, SLC4A4, and Na+-K+ -ATPase were the fluid transport association protein of the corneal endothelium.
Gene |
Sense primer (5′-3′) |
Anti-sense primer (3′-5′) |
Tm (°C) |
Length (bp) |
|---|---|---|---|---|
| K3 | GGCAGAGATCGAGGGTGTC | GTCATCCTTCGCCTGCTGTAG | 56.00 | 398.00 |
| Vimentin | ATGCTTCTTTGGCACGTCTTGACCT | ACTGCACCTGTCTCCGGTATTCGTT | 62.00 | 395.00 |
| NSE | GACAAACAGCGTTACTTAGGCAA | TCGCATGGCATCCCGAAAGC | 60.00 | 393.00 |
| Collagen VIII | ATGTGATGGCTGTGCTGCCT | CTCTTGGGCCAGGCTCTCCA | 62.00 | 408.00 |
| Na+/K+ -ATPase | GGATGATCATAAACTTAGCCTTG | TATAAGCCAAGAAACAAAGAATCG | 56.00 | 224.00 |
| CLCN3 | GTCCATCAATCCATTTGGTAACA | ATAATGACTTCCAGAACGGGATA | 57.00 | 207.00 |
| SLC4A4 | GTTCAGATGAATGGGGATACGC | CGAGCATAAACACAAAGCGTAA | 58.00 | 243.00 |
| VDAC3 | CAGACCCTTCGACCAGGAGT | TTCGCAACCCCTAGACTTCAG | 61.00 | 248.00 |
| GAPDH | GCCAAGGTCATCCATGACAAC | GTCCACCACCCTGTTGCTGTA | 65.00 | 498.00 |
| ACTB | GCACCACTGGTATTGTCATGGA | ATCTTCATGAGGTAGTCCGTCA | 64.00 | 156.00 |