Figure 5. Increased frequency of proliferating HCECs in conditioned medium from mouse embryonic stem cells. A: Passage 4 HCECs were cultured for 48 h and probed for Ki67 expression. B: Ki67-positive cells were analyzed by flow cytometry. Flow cytometry analysis revealed significantly increased Ki67-positive
HCECs in the 25%ESC-CM group (p=0.000). Data are expressed as the mean±SEM (n=3).