Figure 6. Expression of aquaporin 1 in mouse B6-RPE07 cells. A: The presence of aquaporin 1 (AQP1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA expression was detected by reverse-transcriptase PCR using B6-RPE07 cell cDNA (RPE), kidney cDNA (kidney) used as positive control, or nontarget control (CT-). Molecular weight ladders are shown. B: Aquaporin 1 (AQP1) and β-actin (ßactin) protein expressions were detected by western blot in B6-RPE07 cells treated without (Ct) or with 10 ng/ml tumor necrosis factor (TNFα). The blot is representative of three experiments performed using distinct cell preparations.