Figure 5. Inhibition of fibronectin-induced human retinal pigment epithelial (RPE) cell cytoskeletal reorganization by Epigallocatechin gallate (EGCG). Suspended RPE cells pretreated with phosphate buffer saline (PBS, control) or 10 μM EGCG for 1 h were seeded and allowed to adhere on collagen-precoated glass coverslips for an additional 1 h. After fixation, permeabilization, and blocking with 3% BSA (BSA), cells on coverslips were incubated with fluorescein isothiocyanate (FITC)-phalloidin. Mounted cells were analyzed and photographed under a microscope. Actin formed in adherent cells (A), but there was a modification of cytoskeletal reorganization in EGCG-treated cells (B).