Figure 4. Epigallocatechin gallate (EGCG) cannot directly interact with platelet-derived growth factor (PDGF)–BB in dot binding assay. Human recombinant PDGF-BB, phosphate buffer saline (PBS), the indicated concentrations of EGCG, and lycopene (Lyc) were applied onto the nitrocellulose (NC) membrane. The membrane was incubated with PDGF-BB in PBS and then developed by probing with Ab directed against PDGF-BB. Epigallocatechin gallate cannot directly interact with PDGF-BB, but lycopene can. The results presented are representative of three independent experiments.