Figure 2 of Chan, Mol Vis 2010; 16:586-595.

Figure 2. Effects of epigallocatechin gallate (EGCG) on retinal pigment epithelium (RPE) cell migration. A, B: Epigallocatechin gallate inhibits platelet-derived growth factor (PDGF)-BB-induced RPE cell migration. Transwell inserts were coated with fibronectin. Human RPE cells were seeded in the upper chamber in the presence of vehicle or EGCG. The inserts were assembled in the lower chamber, which was filled with serum-free ([−]PDGF-BB; A) or PDGF-BB-containing medium ([+]PDGF-BB; B) and preincubated with a vehicle or EGCG on the polycarbonate filter of the insert for 30 min. PBS, without PDGF-BB or EGCG, served as the control (A, left). Human RPE cells that migrated to the underside of filter membrane were photographed and counted in high-power field (HPF, magnification, 100×) under a phase-contrast light microscope. The scale bar represents 100 μm. The black spots are the pores of the Transwell membrane and the grayish fusiform cells are the ARPE cells. C: Quantitative analysis of migrated cells. Twenty HPFs were counted in each migration. All experiments were conducted in duplicate and similar results were obtained at least two to three times. Data are presented as percent of control (the first unfilled bar, PBS only) in cell counts. *p<0.05 significantly differs from PDGF-BB-stimulated cells (the first filled bar).