Figure 7. Three months after lesioning both central and peripheral injuries caused by photocoagulation (PC) were repaired with new retinal pigmented epithelium (RPE) cells that expressed RPE specific markers. A: Otx2 expression marking RPE cells was continuous across the lesion in whole mounted preparations. The scale bar here represents 100 µm. B: Reveals that repaired regions were hypopigmented with many RPE cells either lacking pigment completely or containing reduced pigment levels. C: Otx2 expression in RPE cells was also continuous across lesions when they were viewed in section. This image shows the location of these cells in relation to the choroidal capillaries (CHO). The scale bar is 25 µm and the image also contains a low power figure of the region. D: The RPE tight junction protein ZO-1 (red) was also present across the lesion site. The nuclei here are shown in blue. Unexpectedly, photoreceptor outer segments (green) remained adhered to the repaired tissue. Their location here demarcates the lesion site. The scale bar here represents 50 µm. E: Shows a high power image of a lesion site revealing Otx2 expression and the brighter cells inside due to the absence of melanin. The scale bar here is 20 µm. F: A transverse section through the repaired region of the RPE. The green outer segments (OS) are indicated by the upper arrow. The lower arrow head reveals the ZO-1 (red). The location of the CHO is also indicated under the other tissues. Nuclei are blue. The scale bar is 20 µm.