Figure 1. In vivo and histological fluorescence images exhibiting the impact of photocoagulation on retinal pigment epithelium. A: In vivo near-infrared reflectance image made immediately after photocoagulation (PC) showing placement of central (arrowhead) and peripheral (arrow) lesions in the retina. Scale bar represents 1 mm. B: In vivo autofluorescence image of a lesion made 7 days after PC shows absence of fluorescence at the center of the lesion. The lesion is surrounded by hyperfluorescent point sources. Scale bar represents 200 µm. C: Autofluorescence image of a lesion in retinal pigment epithelium (RPE), which was excised and flatmounted 6 h after PC. The RPE tissue is continuous across the lesion site, consistent with the notion that PC did not result in immediate tissue destruction. D: Autofluorescence image 72 h after PC, showing a clear lesion site and the absence of tissue. Scale bars in C and D represent 50 µm.