Table 1 of Ma, Mol Vis 2010; 16:556-561. Table 1 of Ma, Mol Vis 2010; 16:556-561.
Table 1. Primers used in polymerase chain reaction for amplification of TGFBI.
Exon |
Primer direction |
Sequence (5′→3′) |
Annealing temperature (°C) |
|---|---|---|---|
| 1 | Forward: | GCTTGCCCGTCGGTCGCTA | 62 |
| Reverse: | TCCGAGCCCCGACTACCTGA | ||
| 2 | Forward: | AGGCAAACACGATGGGAGTCA | 60 |
| Reverse: | TAGCACGCAGGTCCCAGACA | ||
| 3 | Forward: | CCAGATGACCTGTGAGGAACAGTGA | 60 |
| Reverse: | CCTTTTATGTGGGTACTCCTCTCT | ||
| 4 | Forward: | TCCTCGTCCTCTCCACCTGT | 58 |
| Reverse: | CTCCCATTCATCATGCCCAC | ||
| 5 and 6 | Forward: | CCTGGGCTCACGAGGGCTGAGAACAT | 64 |
| Reverse: | GCCCCTCTTGGGAGGCAATGTGTCCC | ||
| 7 | Forward: | GTGAGCTTGGGTTTGGCTTC | 63 |
| Reverse: | ACCTCATGGCAGGTGGTATG | ||
| 8 | Forward: | TGAGGTTATCGTGGAGTG | 53 |
| Reverse: | CACATCAGTCTGGTCACA | ||
| 9 | Forward: | ACTCACGAGATGACATTCCT | 60 |
| Reverse: | TCCAGGGACAATCTAACAGG | ||
| 10 | Forward: | TAGAAGATACCAGATGTTAAGG | 56 |
| Reverse: | TGTCAGCAACCAGTTCTCAT | ||
| 11 | Forward: | CCTGCTACATGCTCTGAACAA | 58 |
| Reverse: | GAATCCCCAAGGTAGAAGAAAG | ||
| 12 | Forward: | GACTCTACTATCCTCAGTGGTG | 58 |
| Reverse: | ATGTGCCAACTGTTTGCTGCT | ||
| 13 | Forward: | CATTAGACAGATTGTGGGTCA | 60 |
| Reverse: | GGGCTGCAACTTGAAGGTT | ||
| 14 | Forward: | GCGACAAGATTGAAACTCCAT | 58 |
| Reverse: | CTCTCCACCAACTGCCACAT | ||
| 15 | Forward: | CCCTCAGTCACGGTTGTT | 58 |
| Reverse: | GGAGTTGCCTTGGTTCTT | ||
| 16 | Forward: | CTTGCACAACTTATGTCTGC | 58 |
| Reverse: | TGCACCATGATGTTCTTATC | ||
| 17 | Forward: | AGTGAAGTTTCACAAACCAC | 58 |
| Reverse: | CCACATTTGGGATAGGTC |