Figure 3. Co-segregation study of members
of family 1 using the HaeIII restriction enzyme. Lane 1 is an
undigested PCR product (286 bp). Lane 2 is a completely digested PCR
product representing the wild-type allele (two products 197 and 56 bp).
Affected individuals (lanes 4, 8, 9, and 10) show the homozygous mutant
allele (one product 253 bp). Unaffected individuals, carriers (lanes 3,
5, 6, and 7) display the mutant allele in a heterozygous state (two
products 253 and 197 bp). M represents the ϕX174RF DNA HaeIII marker.